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Thermo Scientific™ Pierce™ SDS-PAGE Sample Prep Kit

Quickly and easily remove high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis.

Brand:  Thermo Scientific™ 89888


Product Code. 10617514

  • 266.00€ / 1 set
Estimated Shipment: 16-01-2025
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Description

Description

Thermo Scientific™ Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Highlights:

  • Eliminates gel artifacts caused by incompatible contaminants – removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis

  • Compatible with the BCA protein assay – allows protein quantitation of the processed sample before gel-loading

  • Enriches dilute protein solutions – concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes

  • Powerful and flexible – process 2 to 350μL of sample containing up to 70μg of protein; recover 75 to 85% of the total protein in 50μL of elution buffer

  • Convenient, complete kit – includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

    • Includes:

      PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

      TRUSTED_SUSTAINABILITY
Specifications

Specifications

Upon receipt store kit at room temperature.
SDS-PAGE
SDS-PAGE Gels
50 reactions
Sample Loading Buffers
SDS-PAGE Sample Prep Kit
Pierce™
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Safety and Handling

Safety and Handling

Product Identifier
  • Pierce SDS-PAGE Sample Prep Kit

Signal Word
  • Danger

Hazard Category
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity after repeated exposure Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
  • H351-Suspected of causing cancer.
  • H373-May cause damage to organs.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P271-Use only outdoors or in a well-ventilated area.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P314-Get medical advice/attention if you feel unwell.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Kieselguhr, calcined

SDS
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For Research Use Only. Not for use in diagnostic procedures.