Thermo Scientific™ NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer

Description

Thermo Scientific™ TRITC and Rhodamine Labeling Reagents and Kits are high-performance derivatives of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Rhodamine dyes are used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. TRITC, NHS-rhodamine and Texas Red™ Sulfonyl Chloride are reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are often a mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

• Label and purify antibody in about one hour Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification and removal of non-reacted dye with excellent protein recovery