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Thermo Scientific™ Pseudomonas C-N Selective Agar (Cetrimide Agar)

Selectively isolate and differentiate Pseudomonas aeruginosa using Thermo Scientific™ Pseudomonas C-N Selective Agar (Cetrimide Agar) prepared plates.

Brand:  Thermo Scientific™ PO5076A

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Product Code. 11914122

  • 13.55€ / Pack of 10
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Description

Description

Selectively isolate and differentiate Pseudomonas aeruginosa using Thermo Scientific™ Pseudomonas C-N Selective Agar (Cetrimide Agar) prepared plates. Pseudomonas aeruginosa produces a characteristic bright green color on the medium.

Pseudomonas C-N Selective Agar (Cetrimide Agar) is for the selective isolation of Pseudomonas aeruginosafrom a range of samples. 
 
The formula was described by Goto and Enomoto1 following the demonstration of cetrimide as a selective agent by Lowbury and Collins2. 
 
Cetrimide is a quarternary ammonium compound with bactericidal activity against a broad range of Gram-positive organisms and some Gram-negative organisms. 
 
Goto and Enomoto showed that addition of nalidixic acid at 15μg/mL, while at the same time reducing the cetrimide content to 200mg, improved performance. The medium gave better recovery of Pseudomonas aeruginosa with enhanced pigment formation while strongly suppressing Klebsiella, Proteus and Providencia spp., the latter organisms being the troublesome contaminants of conventional cetrimide media.
 
Pseudomonas aeruginosa produces a number of water soluble iron chelators, including the yellow-green or yellow-brown fluorescent pyoverdin. When pyoverdin combines with the blue water-soluble pyocyanin, also produced by the organism, the bright green color characteristic of Pseudomonas aeruginosa is produced. The addition of magnesium chloride and potassium sulphate in the medium enhances the production of these chelators.

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Specifications

Specifications

Oyster white, transparent
Pseudomonas C-N Selective Agar (Cetrimide Agar)
Germany
17 mL
Flow Wrapped - Single Layer
2° to 12°C, in the dark
90mm Monoplate
Agar
17g ± 5%
10/Pk.
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For professional use only (in vitro diagnostic use).