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Rat kidney tissue lysate, Non-denatured; Abnova
Brand: Abnova L039W2.200ug
This item is not returnable.
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Description
Western Blotting, Immunoprecipitation
Specifications
| Immunoprecipitation, Western Blot | |
| Whole tissue lysate (non-denatured) | |
| Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml. | |
| 200 μg | |
| Rat | |
| Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Kidney | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In modified RIPA Lysis Buffer. | |
| 5 mg/mL |