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STEMCELL Technologies ReproRNA™-OKSGM Kit
A non-integrating, self-replicating RNA reprogramming vector for generating iPS cells
Brand: STEMCELL Technologies 05930
This item is not returnable.
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Description
- ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1)
- This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2)
- When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4)
- ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6)
- Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media (mTeSR™1, TeSR™2, or TeSR™-E8™) and further differentiated into cells of all three germ layers (Figure 7)
Specifications
| Pluripotent Stem Cells | |
| Cell Culture; Reprogramming | |
| 1 Kit |