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Thermo Scientific™ SMART Digest™ Trypsin Kits

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

Brand: Thermo Scientific™ 60109-101

757.10 EUR valid until 2025-12-31
Use promo code "25339" to get your promotional price.

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Product Code. 15382360

760.89€ / 1 set

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Beschreibung

Obtain high quality analytical results from protein digests using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

Trypsin Protein Digestion

SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues.

Easy and simple

Significantly increased speed of sample processing
Simple process
Easily automate entire protocol for high-throughput processing

Reliable Results

Increased reproducibility over existing protocols
Higher sensitivity
Higher data confidence

Significantly Reduces Sample Preparation Time

Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.

Cleaner Samples

Reduction in the number of chemicals used and the immobilized trypsin design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. This results in results which are easier to interpret.

Couple with SOLAμ SPE Plates for Desalting

When used with the SMART Digest kits for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.

Spezifikation

Type	Protein Digestion
Format	SMART Digest Resin Pre-filled into PCR Tubes
Cleavage	C-terminal side of lysine and arginine amino acids
Product Line	SMART Digest
Includes	SMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate

Description	SMART Digest Trypsin Kit, with Collection plate
Enzyme	Trypsin
Capture	None
For Use With (Application)	Peptide Quantitation, Peptide Mapping

Produktvorschläge Sicherheit und Handhabung

Sicherheit und Handhabung

Produktidentifikator

Trypsin Kits

Signalwort

Warning

Gefahrenkategorie

Serious eye damage/eye irritation Category 2

Gefahrenhinweis

H319-Causes serious eye irritation.

Sicherheitshinweis

P264-Wash hands thoroughly after handling.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337+P313-If eye irritation persists: Get medical advice/attention.

Ergänzende Informationen

MIXTURE LIST-Contains : sodium azide, calcium chloride

Dokumentation

Scientific Resources

Application Notes

Application Note: A Fast and Simple Immuno-mass Spectrometry Method for Preclinical Bioanalysis for IgG1 mAb File size: 1.2 MB

Application Note: Generic SPE Protocol for Peptide Clean-up and Concentration File size: 703.6 kB

Application Brief: In-Gel Digestion using the SMART Digest Trypsin Kit File size: 270.7 kB

Application Note: Selecting Buffers to Remove Uncertainty in Tryptic Digestion File size: 558.2 kB

Application Note: Impact on Tryptic Digestion when Detergents and Chaotropes are Essential: A Case Study with Ribonuclease A File size: 654.7 kB

Application Note: Rapid Quantitation of Therapeutic Antibodies in Animal Plasma File size: 839.7 kB

Application Note: Fast Digestion Method Optimization Using Innovative Trypsin Technology File size: 812.9 kB

Application Note: Improvement in Speed and Reproducibility of Protein Digestion and Peptide Quantitation, Utilizing Novel Sample Preparation Technology in a Full Solution Workflow File size: 842.2 kB

Application Note: Increased Efficiency of Biomolecule Identification by Optimization of Trypsin Digestion Buffers File size: 633 kB

Application Note: Enhanced Digestion for Improved Biomarker Identification File size: 449.4 kB

Brochures

Brochure: SOLA Solid Phase Extraction Products – Consistent Excellence for Bioanalysis File size: 8.1 MB

Technical Guide: SOLA SPE Method Development Guide - Method Optimization for SPE Bioanalysis File size: 5.9 MB

SMART Digest and SMART Digest ImmunoAffinity (IA) Kit Technical Guide – Smarter Protein Preparation File size: 9.1 MB

Brochure: Protein Digestion for Peptide Mapping and Quantitation: Overview of SMART Digest and SMART Digest ImmunoAffinity Kits File size: 6 MB

Peptide Mapping – Sample to Knowledge Easier and Faster than Before File size: 4.2 MB

Technical Guide: SOLAμ Solid Phase Extraction (SPE) Well Plates – Consistent Excellence for Bioanalysis File size: 1.1 MB

SMART Digest Soluble Trypsin Kit – Smarter Protein Digestion FAQ File size: 1,015 kB

Flyer: Presentation: Improved Performance in Capillary GC Columns File size: 2.4 MB

Brochure: SMART Digest File size: 7.2 MB

Brochure: SMART Digest Kit Technical Guide File size: 3.5 MB

Flyers

Flyer: The Right Columns and Consumables to Maximize your Pharmaceutical Workflow Version: 0419S File size: 327 kB

Flyer: FAQ: SMART Digest Kit – Facilitating Perfect Digestion File size: 2.4 MB

Posters

Improvement in Speed and Reproducibility of Protein Digestion Utilizing Novel Sample Preparation Technology in a Full Solution Workflow File size: 1.5 MB

Poster Note: Generation of a Novel Digestion Protocol for Enhanced Proteome Coverage File size: 823.7 kB

Product Information

Manuals

SMART Digest Kit – User Manual (Revision B) Version: DEC.2017 File size: 8.2 MB

SMART Digest ImmunoAffinity (IA) Kit – User Manual (Revision A) Version: SEP.2016 File size: 8.5 MB

Sonderangebote

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