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IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.
Brand: IBA Lifesciences 5-4544-001
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Description
pESG-IBA mammalian expression vectors
pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.
- NeoR for generation of stable cells lines.
- Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- ColE1 + Amp: for propagation in E. coli
- NeoR: neomycin resistance gene to generate stable cell lines
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
pESG-IBA mammalian expression vectorspESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.
- NeoR for generation of stable cells lines.
- Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- ColE1 + Amp: for propagation in E. coli
- NeoR: neomycin resistance gene to generate stable cell lines
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifications
| Liquid | |
| pESG-IBA 144 | |
| 8 | |
| Acceptor Vector for mammalian expression | |
| 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| Twin-Strep-tag for N-terminal | |
| 5 μg | |
| Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins |
| StarGate™ System, Mammalian Host Cells | |
| SV40 large T antigen (e.g. COS1 or COS7) | |
| Human CMV Immediate-early | |
| 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg | |
| Mammalian | |
| 250 ng/μL | |
| Clear |