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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression
Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.
220.00€ - 266.00€
Specifications
| Format | Liquid |
|---|---|
| For Use With (Equipment) | StarGate™ System, Yeast Host Cells |
| Inducing Agent | Copper sulfate |
| Marker Gene | leu2-d and URA3 |
| pH | 8 |
| Product Code | Brand | Description | Protein Tag | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Description | Protein Tag | Price | Quantity & Availability | ||||
|
15613808
|
IBA Lifesciences
5-4600-001 |
pYSG-IBA wt1 | Untagged |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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|
15623808
|
IBA Lifesciences
5-4603-001 |
pYSG-IBA 3 | Strep-tag for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15633808
|
IBA Lifesciences
5-4605-001 |
pYSG-IBA 5 | Strep-tag for N-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15643808
|
IBA Lifesciences
5-4623-001 |
pYSG-IBA 23 | Strep-tag for C-terminal and GST-tag for N-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15653808
|
IBA Lifesciences
5-4625-001 |
pYSG-IBA 25 | GST Tag for N-terminal | N/A | |||||
|
15663808
|
IBA Lifesciences
5-4633-001 |
pYSG-IBA 33 | His Tag (6x) for C-terminal | N/A | |||||
|
15673808
|
IBA Lifesciences
5-4643-001 |
pYSG-IBA 43 | His Tag (6x) for N-terminal and Strep-tag for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15683808
|
IBA Lifesciences
5-4645-001 |
pYSG-IBA 45 | Strep-tag for N-terminal and His Tag (6x) for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15693808
|
IBA Lifesciences
5-4662-001 |
pYSG-IBA 62 | Strep-tag for C-terminal and FLAG-tag for N-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15603818
|
IBA Lifesciences
5-4663-001 |
pYSG-IBA 63 | FLAG-tag for C-terminal | N/A | |||||
|
15613818
|
IBA Lifesciences
5-4664-001 |
pYSG-IBA 64 | Strep-tag for N-terminal and FLAG-tag for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15623818
|
IBA Lifesciences
5-4665-001 |
pYSG-IBA 65 | FLAG-tag for N-terminal | N/A | |||||
|
15633818
|
IBA Lifesciences
5-4703-001 |
pYSG-IBA 103 | Twin-Strep-tag for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15643818
|
IBA Lifesciences
5-4705-001 |
pYSG-IBA 105 | Twin-Strep-tag for N-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15653818
|
IBA Lifesciences
5-4723-001 |
pYSG-IBA 123 | Twin-Strep-tag for C-terminal and GST-tag for N-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15663818
|
IBA Lifesciences
5-4743-001 |
pYSG-IBA 143 | His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15673818
|
IBA Lifesciences
5-4745-001 |
pYSG-IBA 145 | Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15683818
|
IBA Lifesciences
5-4762-001 |
pYSG-IBA 162 | Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15693818
|
IBA Lifesciences
5-4764-001 |
pYSG-IBA 164 | Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
|
15603828
|
IBA Lifesciences
5-4767-001 |
pYSG-IBA 167 | Twin-Strep-tag/FLAG-tag for N-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
Log in to see stock. |
||||
|
15613828
|
IBA Lifesciences
5-4768-001 |
pYSG-IBA 168 | Twin-Strep-tag/FLAG-tag for C-terminal |
266.00€
5µg |
Estimated Shipment: 10-12-2024
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||||
Beschrijving
pYSG-IBA yeast expression vectorsThe pYSG-IBA vector allows the high-level expression of proteins in yeast.
- The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
- The expressed recombinant protein will be localized in the cytoplasm.
- High-level expression in yeast with high copy number
- Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
- Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
- URA3 auxotrophy marker for selection after transformation
Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specificatie
| Liquid | |
| Copper sulfate | |
| 8 | |
| Acceptor Vector for yeast expression | |
| 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| 5 μg | |
| Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins |
| StarGate™ System, Yeast Host Cells | |
| leu2-d and URA3 | |
| CUP1 | |
| 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg | |
| 250 ng/μL | |
| Clear |
Veiligheid en behandeling
missing translation for 'shelfLife' : 12 months