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Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
Brand: Thermo Scientific™ 34075
323.05 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.
Description
Thermo Scientific™ SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.
SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems and quantitation. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, enabling multiple film or camera exposures.SuperSignal West Dura Extended Duration Substrate characteristics include:
- Sensitivity: mid-femtogram
- Stability: 24-hr working solution stability; 1-year kit stability at room temperature
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: 24 hours
- Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
- Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Specifications
Substrate | |
Liquid | |
Nitrocellulose, PVDF | |
100 mL | |
2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL) | |
24 hr. | |
Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL |
Chemiluminescence | |
Western Blot | |
SuperSignal™ | |
Horseradish Peroxidase | |
Mid-femtogram | |
12 Mini-Gel Blots, 1000 sq. cm of Membrane | |
Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane • Luminol/Enhancer, 50 mL • Stable Peroxide Buffer, 50 mL Store at room temperature. |
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Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
ZK400245 | Certificate of Analysis | 16/12/2024 | 34075,34075_LITL18 PROMO |
ZK400245A | Certificate of Analysis | 16/12/2024 | 34075,34075_LITL18 PROMO |
ZI397732 | Certificate of Analysis | 16/10/2024 | 34075,34075_LITL18 PROMO |
ZH394561 | Certificate of Analysis | 04/09/2024 | 34075,34075_LITL18 PROMO |
ZH394561A | Certificate of Analysis | 04/09/2024 | 34075,34075_LITL18 PROMO |
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For Research Use Only. Not for use in diagnostic procedures.