Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay

Description

Rapidly detect multiple genetic targets for Shiga-toxin producing Escherichia coli O157:H7 and other STEC in food and environmental samples with the Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC PCR workflow. The workflow has been granted AOAC Performance Tested Methods^SM Certification from the AOAC Research Institute and NF VALIDATION™ from AFNOR (ISO 16140-2:2016).

The assay has been extensively tested on a range of food matrices from food manufacturing environments, including raw meat (excluding poultry), vegetables, fruits, juices, and raw dairy samples and enables users to reliably detect and differentiate Shiga-toxin producing Escherichia coli O157:H7 and other STEC in as little as 10 hours.

The SureTect PCR System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.

The Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay is part of a complete workflow for the rapid detection of Escherichia coli O157:H7, stx, eae, and the Escherichia coli STEC serotypes (O26, O45, O103, O111, O121, O145) from the tested samples. 

Samples assessed as positive using the SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay are further tested using this SureTect™ Escherichia coli STEC Identification PCR Assay.
 

Simple

• Easy-to-use with single enrichment step and pre-dispensed reagents
• Target-specific colored assay plastics
• Clearly oriented lysis and assay trays
• Color indicator in lysis reagent reducing possibility for human error

Fast and Flexible

• Option to automate the lysis and PCR sample preparation
• Optimized workflow delivers on-screen results in as little as 10 hours
• Maximum flexibility for operational efficiency
• Run all assays simultaneously in the same run
• Test up to 96 samples in each run
• Choice of pierceable seals or caps
• Detect multiple targets in the same run with universal PCR protocols across the range

Smart

• Intuitive, user-friendly software delivers reliable results, with no subjective interpretation
• Actionable data supports fast decisions in a complex product supply chain
• IPC in every assay for complete confidence in results

Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay includes:

• Lysis Reagent 1 Tubes
• Proteinase K
• Escherichia coli O157:H7 and STEC Screening PCR Tubes
• Thermo Scientific 96-Well Pierceable Seals
• PCR Caps

Enriched samples are pipetted into pre-filled lysis tubes, along with Proteinase K, before incubation, to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Escherichia coli O157:H7 and STEC Screening PCR tubes to re-hydrate the pre-loaded PCR pellet which contains all the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal positive control (IPC).

The PCR tubes are sealed, then loaded into the Applied Biosystems™ QuantStudio™ 5 Food Safety Real Time PCR Instrument with RapidFinder™ Analysis Software v3.0 or later. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required. The internal amplification control confirms correct assay conditions have occurred.

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