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Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Brand: Thermo Scientific™ EL0016

57.33 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Product Code. 10786591

82.00€ / 2 units

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Description

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

The T4 DNA Ligase requires ATP as a cofactor.

Highlights

Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
Fast – sticky-end ligation is completed in 10 minutes at room temperature
Supplied with PEG solution for efficient blunt-end ligation

Applications

Cloning of restriction enzyme generated DNA fragments
Cloning of PCR products
Joining of double-stranded oligonucleotide linkers or adaptors to DNA
Site-directed mutagenesis
Amplified fragment length polymorphism (AFLP)
Ligase-mediated RNA detection (see Reference 3)
Nick repair in duplex DNA, RNA or DNA/RNA hybrids
Self-circularization of linear DNA.

Notes

Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture by spin column or chloroform extraction. The extracted DNA can be further precipitated with ethanol.

Specifications

Concentration	1 U/μL
Compatible Buffer	10X T4 DNA Ligase Buffer
Product Type	T4 DNA Ligase

Enzyme	DNA Ligase
Quantity	2 x 500 units

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

T4 DNA Ligase, LC (1 U/µL)

Signal Word

Warning

Hazard Category

Serious eye damage/eye irritation Category 2

Hazard Statement

H319-Causes serious eye irritation.

Precautionary Statement

P264-Wash hands thoroughly after handling.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

MIXTURE LIST-Contains : (R*,R*)-1,4-dimercapto-2,3-butanediol

Documents

Product Information

Manuals

User Guide: Linker Ligation Version: Jan. 2015 File size: 73.5 kB

User Guide: Control Reaction for T4 DNA Ligase Activity Version: Jan. 2015 File size: 83.8 kB

User Guide: Self-circularization of Linear DNA Version: Jan. 2015 File size: 70 kB

User Guide: DNA Insert Ligation (sticky and blunt-end) into Vector DNA Version: May 2015 File size: 81 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2851702	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberEL0016
Lot Number2851715	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberEL0016
Lot Number2952600	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberEL0016
Lot Number2952521	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberEL0016
Lot Number3066671	Certificate TypeCertificate of Analysis	Date25/01/2025	Catalog NumberEL0016

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Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL) > 1μ/μL, 2 x 500U

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