Applied Biosystems™ TaqMan™ Advanced miRNA Human Endogenous Controls Card

Description

This product contains one card than can be used to evaluate 30 potential endogenous control miRNAs in triplicate for up to four samples. Two exogenous (non-human) controls are included for normalization of sample input.

Features of the TaqMan Advanced miRNA Human Endogenous Controls Card include:
• Reproducibility—pre-spotted assays help reduce pipetting error and minimize experimental variability
• Sample savings—requires only 2 μL purified plasma/serum or 1–10 ng total RNA per sample
• Updated content—assay content updated for mirBase v.22
• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:

Number of cards: 1
Number of unique miRNAs (not including exogenous controls): 30
Exogenous controls: cel-miR-39-3p, ath-miR159a
Compatible with the following Applied Biosystems qPCR instruments: Viia7, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA cards require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses a miRNA-specific reverse transcription chemistry.