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  5. Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent
  6. 15255743

Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

Brand:  Thermo Scientific™ 89821

356.01 EUR valid until 2025-03-21
Use promo code "24111" to get your promotional price.

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Product Code. 15255743

  • 534.00€ / 250mL
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Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
This item is not returnable. View return policy

Description

Description

  • An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
  • All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
  • This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
  • Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

  • Detergent in Tris buffer with Lysozyme and Universal Nuclease
  • Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
  • Recovery of large molecular weight proteins (high MW proteins)
  • Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
  • More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Bacterial Protein Extraction Reagent
Store at 4°C.
250 mL
250 mL
B-PER™
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Lot Number Certificate Type Date Catalog Number
Lot NumberAA403817 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog Number89821
Lot NumberZL403480 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog Number89821
Lot NumberZK399422 Certificate TypeCertificate of Analysis Date16/12/2024 Catalog Number89821
Lot NumberZJ399421 Certificate TypeCertificate of Analysis Date30/10/2024 Catalog Number89821
Lot NumberZI399420 Certificate TypeCertificate of Analysis Date15/10/2024 Catalog Number89821
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Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent > 250 mL

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