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Invitrogen™ Thyroglobulin Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHTGX5
This item is not returnable.
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Description
The Human Thyroglobulin (TG) ELISA quantitates Hu TG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TG. Principle of the method The Human TG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The human thyroglobulin (hTg) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are,.
Specifications
| P01266 | |
| 220 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Plasma, Serum, Supernatant | |
| 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 7038 | |
| <12% | |
| Wash Buffer, Assay Diluent concentrate, Standard, SAV-HRP, Stop Solution, Pre-coated 96 well plate, Adhesive Plate Covers, Biotinylated Detection Antibody, Chromogen | |
| 5 x 96 Tests | |
| Endocrinology | |
| 2°C to 8°C | |
| Human | |
| 4 hr. 45 min. |
| 0.205 to 50 ng/mL | |
| 0.22 to 50 ng/mL | |
| Biotin | |
| Hormones & Receptors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| Human | |
| Colorimetric Microplate Reader | |
| AITD3N,TG | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 50 uL; Serum, 50 uL; Supernatant, 100 uL | |
| AITD3N, TG | |
| 1 hr. 20 min. |