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Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
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Format | Spin Column |
Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
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Polymerase | Phusion High-Fidelity DNA Polymerase |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
GC-Rich PCR Performance | High |
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Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Label or Dye | SYBR Green |
Product Line | SYBR™ |
This plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems.
Satisfies the same requirements as the real-time PCR system installation.
Thermo Scientific dATP (2'-deoxyadenosine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
Shipping Condition | Dry Ice |
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Purity or Quality Grade | >99% |
Form | Liquid |
Label or Dye | Unlabeled |
Concentration | 10 mM |
Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 10 X |
Reverse Transcriptase | MultiScribe™ |
Final Product Type | cDNA |
Concentration | 10X |
For Use With (Application) | miRNA analysis |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Size (Final Product) | 200 bp or less |
Reaction Format | Separate Components |
Product Type | MicroRNA Reverse Transcription Kit |
Sample Type | RNA |
Reaction Speed | Fast |
Optimal Reaction Temperature | 42°C |
Product Line | TaqMan™ |
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.
Polymerase | DreamTaq DNA Polymerase |
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GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 5 U/μL |
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
Shipping Condition | Dry Ice |
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Polymerase | Phusion Flash II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Color | Colorless |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |