Protein Biology Methods
Genes direct the production of proteins that play a significant role in diseases such as cancer, Alzheimer’s, and diabetes. Protein biology helps us understand the output of these genes and the way they influence our health. Through protein isolation, purification, separation, and detection, protein biology provides earlier and more accurate diagnoses.
Step 1: Extraction
Isolate proteins from your mixture with the products below. This protein biology method will help you start your process and prepare you for future workflow steps. Find protein extraction essentials, lysis reagents, plus additional products and resources.
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Featured Products
Catalog Number 13463119
Invitrogen™ Total Exosome RNA & Protein Isolation Kit
Catalog Number 4531883
Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent
Catalog Number 4919324
Gibco™ TrypLE™ Express Enzyme (1X), no phenol red
Resources
Pall AcroPrep 24 Well Filter Plates Application Note
- Filename
- Pall_AcroPrep_24_Well_Filter_Plates_Application_Note.pdf
- Size
- 2 MB
- Format
- application/pdf
Pall AcroPrep 24 Well Filter Plate Family
- Filename
- Pall_AcropPrep_24_Well_Filter_Plate_Family.pdf
- Size
- 2 MB
- Format
- application/pdf
Pall Centrifugal Devices
- Filename
- Pall_Centrifugal_Devices.pdf
- Size
- 3 MB
- Format
- application/pdf
Benchtop Instrument Portfolio
- Filename
- Benchtop_Instrument_Portfolio.pdf
- Size
- 2 MB
- Format
- application/pdf
Step 2: Clean Up
Once extracted, the sample needs to be cleaned of the impurities that can hinder downstream sample analysis. We offer the full clean up workflow, from dialysis to desalting and concentration.
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Featured Products
Catalog Number 10415545
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Catalog Number 2410196
Spectrum™ Spectra/Por™ Float-A-Lyzer™ G2 Dialysis Devices
Catalog Number 11345402
Thermo Scientific™ Pierce™ Protein Concentrators PES, 3K or 5K MWCO, 0.5–100 mL
Resources
Thermo Scientific™ Pico™ and Fresco™ Microcentrifuge Series
- Filename
- Thermo_Scientific_Pico_and_Fresco_Microcentrifuge_Series.pdf
- Size
- 21 MB
- Format
- application/pdf
Eppendorf™ Catalog 2022 EN
- Filename
- Eppendorf_Catalogue_2022_English.pdf
- Size
- 22 MB
- Format
- application/pdf
Step 3: Purification
Purification is another necessary protein biology method and involves separation by protein binding, washing, and elution. The protocol used will depend on the protein you’re purifying. To get started, shop prepacked columns, products for protein purification, dialysis and desalting, and endotoxin removal. For further information, download our resources.
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Featured Products
Catalog Number 11859400
Thermo Scientific™ Pierce™ Protein A/G UltraLink™ Resin
Catalog Number 15672135
Thermo Scientific™ POROS™ HQ 50 μm Strong Anion Exchange Resin
Catalog Number 3676634
Cytiva HisTrap™ HP Prepacked Columns
Resources
Thermo Scientific™ Pierce™ Protein and Peptide Assay Selection Guide
- Filename
- Pierce protein and peptide assay selection guide.pdf
- Size
- 4 MB
- Format
- application/pdf
Protein Assay Technical Handbook
- Filename
- Protein_Assay_Technical_Handbook.pdf
- Size
- 13 MB
- Format
- application/pdf
IBA Protein Production and Assays
- Filename
- IBA_Protein_Production_And_Assays.pdf
- Size
- 4 MB
- Format
- application/pdf
Step 4: Separation
Solubility, size, charge, and other protein characteristics are used to separate them. These techniques include various forms of chromatography in addition to electrophoresis and dialysis. Find the products you need for each of these methods and more by browsing gels, columns, and reagents. Learn more by downloading our relevant resources.
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Featured Products
Catalog Number 7182048
Invitrogen™ Novex™ Tris-Glycine Mini Protein Gels, 4–12%, 1.0 mm, WedgeWell™ format
Catalog Number 11832124
Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
Catalog Number 15824992
Invitrogen™ Novex™ Tricine Welcome Pack, 16%
Resources
Unique Protein Gel for Your Every Need
- Filename
- Unique_Protein_Gel_for_your_every_Need.pdf
- Size
- 7 MB
- Format
- application/pdf
Step 5: Protein Detection
Western blotting is frequently used for protein detection. Browse related reagents, substrates, and imaging systems to help advance your protein detection protocol.
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Featured Products
Catalog Number 6911011
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Catalog Number 8903001
Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
Catalog Number 17102886
Invitrogen™ Bandmate™ Automated Western Blot Processor
Resources
Bandmate Automated Western Blot Processor
- Filename
- Bandmate_Automated_Western_Blot_Processor.pdf
- Size
- 686 KB
- Format
- application/pdf
BOOST Your DNA Analysis
- Filename
- BOOST_your_DNA_analysis.pdf
- Size
- 544 KB
- Format
- application/pdf
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