Modifying Enzymes

Modifying Enzymes
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Thermo Scientific™ RNase A/T1 Mix
Get the RNA degradation activity of both RNase A and RNase T1 with Thermo Scientific™ RNase A/T1 Mix.

Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Product Type | T4 DNA Ligase |
---|---|
Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Content And Storage | -20°C |
---|---|
Form | Solid |
Product Type | Proteinase K |
Color | White |
For Use With (Application) | Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases |
Source | Tritirachium album |
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.

Product Type | RNase H |
---|---|
Enzyme | RNase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 5 U/μL |
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Form | Liquid |
---|---|
Product Type | Proteinase K |
Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

Product Type | RNase Inhibitor |
---|---|
Compatible Buffer | Storage Buffer |
Concentration | 40 U/μL |
Product Line | RiboLock™ |
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Product Type | Exonuclease I |
---|---|
Enzyme | Exonuclease |
Concentration | 20 U/μL |
Thermo Scientific™ Micrococcal Nuclease (300 U/μL)
Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.

Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.

Content And Storage | Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C. |
---|---|
Product Type | Uracil DNA Glycosylase |
Enzyme | Uracil-DNA Glycosylase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 1 U/μL |
Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Thermo Scientific™ RNase T1 (1000 U/μL)
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Product Type | RNase T1 |
---|---|
Enzyme | RNase |
Concentration | 1000 U/μL |