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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
High-quality, calibrated BSA solutions (2 mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
Shipping Condition | Room Temperature |
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Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
Conjugate | HRP |
Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 24 hr. |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Brown-to-purple (A595nm), ready-to-use, fast reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Product Type | DTT (Dithiothreitol) |
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Concentration | 0.1 M |
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X TriTrack DNA Loading Dye.
Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.
Assay | BCA Assay |
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Specificity | Not Target-Specific |
Content And Storage | Store at room temperature. |
Description | Pierce BCA Protein Assay Reagent A |
For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Substrate | Horseradish Peroxidase |
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Form | Liquid |
Product Type | Substrate |
Signal Duration | 6 to 8 hr. |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent used to quickly measure total protein concentration compared to a protein standard.
Assay | Bradford Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
Sufficient For | 630 Tube Assays or 3160 Microplate Assays |
Quantity | 950 mL |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
Precipitate DNA and RNA with this 0.22ÎĽm membrane-filtered, sterile, nuclease-free solution of sodium acetate.