Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Thermo Scientific™ Pierce™ Protein-Free Blocking Buffer
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.
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Thermo Scientific™ Pierce™ Recombinant Protein A/G, Peroxidase Conjugated
Detect antibodies, especially IgG isotypes, from many different species hosts with this purified, alkaline phosphatase- or peroxidase-conjugated recombinant Protein A/G.
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Thermo Scientific™ Pierce™ Recombinant Protein A/G
Detect antibodies, especially IgG isotypes, from many different species hosts with this purified, alkaline phosphatase- or peroxidase-conjugated recombinant Protein A/G.
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Thermo Scientific™ Pierce™ Recombinant Protein A
Use Thermo Scientific Pierce Recombinant Protein A to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
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Thermo Scientific™ Pierce™ Recombinant Protein L
Detect mouse and human antibodies containing kappa light chains in immunoassays using this purified, biotinylated or peroxidase-conjugated, recombinant Protein L.
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Thermo Scientific™ Pierce™ Recombinant Protein G
Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.
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Thermo Scientific™ Pierce™ Protein A
Detect rabbit and human antibodies, especially IgG isotypes, for Western blot, ELISA and other immunoassays with this purified and biotinylated recombinant Protein A.
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Thermo Scientific™ Pierce™ Protein A, Biotinylated
Detect rabbit and human antibodies, especially IgG isotypes, for Western blot, ELISA and other immunoassays with this purified and biotinylated recombinant Protein A.
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Thermo Scientific™ Pierce™ Recombinant Protein A, Peroxidase Conjugated
Detect rabbit and human antibodies, especially IgG isotypes, in Western blot, ELISA and other immunoassays with this purified and peroxidase-conjugated recombinant Protein A.
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Thermo Scientific™ Pierce™ Recombinant Protein L, Biotinylated
Detect mouse and human antibodies containing kappa light chains in immunoassays using this purified, biotinylated or peroxidase-conjugated, recombinant Protein L.
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Thermo Scientific™ Pierce™ Recombinant Protein G, Biotinylated
Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.
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Thermo Scientific™ Pierce™ Recombinant Protein L, Peroxidase Conjugated
Detect mouse and human antibodies containing kappa light chains in immunoassays using this purified, biotinylated or peroxidase-conjugated, recombinant Protein L.
Promotions are available
Thermo Scientific™ Pierce™ Recombinant Protein G, Peroxidase Conjugated
Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.
BD Protein A: HRP
For routine western blotting analysis