Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

1 – 15 of 34 results

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

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Specifications

Format	Spin Column
Run Time	5 min.
High-throughput Compatibility	Not High-throughput Compatible (Manual)

First Strand cDNA Synthesis Kit

Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.

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Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

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Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Medium
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR
Polymerase	Phire Hot Start II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Tissue
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

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Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

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Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

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Specifications

Product Type	Multiplex Kit
For Use With (Equipment)	3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type	DNA
Technique	SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line	SNaPshot™
Detection Method	Primer-probe

Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

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Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phusion Blood II DNA Polymerase
GC-Rich PCR Performance	High
Reaction Format	Separate Components
Product Type	Direct PCR Kit
Sample Type	Blood
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2 U/μL
Starting Material	DNA
PCR Method	Direct PCR

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

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Thermo Scientific™ MuA Transposase (concentrated)

Perform functional analysis of proteins with these transposon-based tools.

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Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

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Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	High
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	Direct PCR
Polymerase	Phusion Blood II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Blood
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

Verso™ 1-Step SYBR Green-Based Detection RT-qPCR Kits

Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits have been specially developed to quantify RNA in a convenient one-step assay.

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Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)

Thermo Scientific™ Maxima™ 5X Reaction Mix is a component of the Maxima First Strand cDNA Synthesis Kits for RT-qPCR (K1671/K1672/K1641/K1642) and may be purchased separately. The 5X Reaction Mix is comprised of reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers.

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