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Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
Shipping Condition | Dry Ice |
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Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 2X |
For Use With (Application) | Hot-start PCR |
Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
Polymerase | DreamTaq DNA Polymerase |
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GC-Rich PCR Performance | Low |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | Separate Components |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
HotTaq PCR mix is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template.
Kit for rapid mouse typing experiments with common samples such as tail clips, ear punch, hair, and blood.
Activation Time | Lysis 2 min; Enzyme inactivation 3 min; PCR cycling 30–90 min |
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Content And Storage | NucleoType HotStart PCR Master Mix (2x), Lysis Buffer M, Proteinase K. Store at 4 °C or -20 °C |
Format | Kit |
Size (Final Product) | Amplicon size: up to 1000 bp |
Sample Type | Mouse tail clipping (1 mm), mouse ear punch (Dia. 1 mm), mouse blood (1 μL), mouse hairs (approx. 3–30) |
Yield | 100 - 600 ng amplicon per 10 μL PCR |
Shelf Life | 1 year |
For Use With (Application) | Genotyping |
Detection Method | Primer-probe |
Time and money saving seed genotyping workflow.
Content And Storage | Lysis Buffer P; Proteinase K; NucleoType HotStart PCR Master Mix (2x) containing polymerase, dNTPs, buffer, enhancer, loading dye and stabilizer. Store at 4°C or -20°C |
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Format | Kit |
Size (Final Product) | Amplicon size up to 2000 bp |
Sample Type | Hard plant material like e.g., seeds from soybeen, wheat, corn, rice, as well as from moss, fern leaf, and fir needle |
Reaction Speed | 30 to 90 min. |
Kit Type | Simple sample preparation suitable for hot start PCR |
For Use With (Application) | Genotyping |
Enhance the specificity, sensitivity, and yield of DNA amplification with this hot start DNA polymerase.
Hot-start modified for PCR applications requiring maximum specificity and sensitivity to detect rare or complex targets and allow for room-temperature reaction setup.
Thermo Scientific™ Phire Hot Start DNA Polymerase is the older version of Phire Hot Start DNA Polymerase; for upgraded version, please see Phire Hot Start II DNA Polymerase.
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps
Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps