All Primary Antibodies

All Primary Antibodies
Primary antibodies, immunoglobulins that bind to specific proteins or other biomolecules, are used in many research applications and protocols to detect targets of interest. They are developed using different animal hosts, including mouse, rat, rabbit, goat, sheep, and many others.
Monoclonal and Polyclonal Antibodies
One type of primary antibodies, monoclonal antibodies, provides high reproducibility and low cross-reactivity and background noise. Another type, polyclonal antibodies, often costs less and provides greater affinity and quicker binding. Both are produced using plasma B cells, but the former uses the same clone and the latter uses different clones. Monoclonal antibodies require hybridoma cell lines, and polyclonal antibodies do not.
There are also recombinant monoclonal antibodies with similar benefits, like high affinity, scalability, and specificity. They are produced using in vitro cloning of plasma B cells and expression hosts.
Conjugated Primary Antibodies
Antibodies can be labeled with various fluorophores or detection agents or used without labels. Labeled primary antibodies, also known as conjugated primary antibodies, help researchers simplify and streamline their applications. They are coupled with common enzymes and dyes such as Alexa Fluor and often used in protein and cell analysis.
Applications
Antibodies for life sciences applications are used in flow cytometry, western blotting, ELISA, immunohistochemistry, and immunocytochemistry. Secondary antibodies can be added to support the detection and purification of certain antigens. They bind to the primary antibody, which binds to the antigen of interest. Finding the right combination of antibodies can result in greater antigen specificity and a strong, detectable signal.
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Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
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Target Species | Mouse |
Host Species | Rat |
Conjugate | Unconjugated |
Applications | Western Blot,Immunoprecipitation |
Form | Purified |
Isotype | IgG2a |
Gene Accession No. | O08677 |
Concentration | LYOPH |
Antigen | Kininogen |
Gene Symbols | Kng1 |
Regulatory Status | RUO |
Purification Method | Protein A or G purified from hybridoma culture supernatant |
Dilution | Western Blot 1 ug/mL, Immunoprecipitation 25 ug/mL |
Gene ID (Entrez) | 3827 |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Kininogen Glu21-Ser661 Accession # O08677 |
Classification | Monoclonal |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
Primary or Secondary | Primary |
Test Specificity | Detects mouse Kininogen Light Chain in direct ELISAs and Western blots. In Western blots, reacts with full length Kininogen (aa 21-661) and several forms of the light chain starting at residue 389, and does not react with the heavy chain. In Western blots, no cross-reactivity with recombinant mouse (rm) Cystatin A, B, C, E/M, recombinant human (rh) Cystatin D, F, S, SA, SN, rmCystatin/Stefin Homolog, rmFetuin A, rhFetuin B, rmHPRG, rhKininogen, or rhKininostatin is observed. |
Clone | 288424 |
Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
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Target Species | Human |
Host Species | Goat |
Conjugate | Unconjugated |
Applications | Western Blot,Immunoprecipitation |
Isotype | IgG |
Gene Accession No. | P01042 |
Concentration | LYOPH |
Antigen | Kininogen |
Gene Symbols | KNG1 |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Dilution | Western Blot 0.1 ug/mL, Immunoprecipitation 25 ug/mL |
Gene ID (Entrez) | 3827 |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Kininogen Gln19-Ser644 Accession # P01042 |
Classification | Polyclonal |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. |
Primary or Secondary | Primary |
Test Specificity | Detects human Kininogen in direct ELISAs and Western blots. |
Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
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Target Species | Human |
Host Species | Mouse |
Conjugate | Unconjugated |
Applications | Western Blot,Immunoprecipitation |
Form | Purified |
Isotype | IgG2b |
Gene Accession No. | P01042.2 |
Concentration | LYOPH |
Antigen | Kininogen |
Gene Symbols | KNG1 |
Regulatory Status | RUO |
Purification Method | Protein A or G purified from hybridoma culture supernatant |
Dilution | Western Blot 1 ug/mL, Immunoprecipitation 25 ug/mL |
Gene ID (Entrez) | 3827 |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Kininogen Gln19-Ser644 (Ile581Thr) (predicted) Accession # P01042.2 |
Classification | Monoclonal |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
Primary or Secondary | Primary |
Test Specificity | Detects human Kininogen Light Chain in direct ELISAs and Western blots. In Western blots, it reacts with the full length (amino acids 19-644) and two forms of the light chain starting at amino acids 390 and 438, respectively, and does not react with the E. coli-expressed D5/Kininostatin. In Western blots, no cross-reactivity with recombinant human (rh) Cystatins A, B, C, D, E/M, F, S, SA, SN, rhFetuins A, B, rhHPRG, or recombinant mouse Kininogen is observed. |
Clone | 236006 |
Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
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Target Species | Human |
Host Species | Mouse |
Conjugate | Unconjugated |
Applications | Western Blot,Immunoprecipitation |
Form | Purified |
Isotype | IgG1 |
Gene Accession No. | P01042 |
Concentration | LYOPH |
Antigen | Kininogen |
Gene Symbols | KNG1 |
Regulatory Status | RUO |
Purification Method | Protein A or G purified from hybridoma culture supernatant |
Dilution | Western Blot 1 ug/mL, Immunoprecipitation 25 ug/mL |
Gene ID (Entrez) | 3827 |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Kininogen Gln19-Ser644 (Ile581Thr) Accession # P01042 |
Classification | Monoclonal |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
Primary or Secondary | Primary |
Test Specificity | Detects human Kininogen Heavy Chain in direct ELISAs and Western blots. It reacts with HK, Kininogen, and the heavy chain, but not with the light chain alone. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E, S, SA, SN, rhFetuin A, rhFetuin B, rhHPRG, recombinant mouse Kininogen, or rhKininostatin is observed. |
Clone | 236012 |
Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
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Target Species | Mouse |
Host Species | Rat |
Conjugate | Unconjugated |
Applications | Immunohistochemistry |
Form | Purified |
Isotype | IgG2a |
Gene Accession No. | Q62426 |
Antigen | Cystatin B/Stefin B |
Gene Symbols | CSTB |
Regulatory Status | RUO |
Purification Method | Protein A or G purified from hybridoma culture supernatant |
Dilution | Immunohistochemistry 8-25 ug/mL |
Gene Alias | CPI-B, CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B, CSTB, cystatin B (stefin B), EPM1, Liver thiol proteinase inhibitor, PME, stefin B, stefin-B |
Gene ID (Entrez) | 1476 |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative |
Immunogen | E. coli-derived recombinant mouse Cystatin B Met2-Phe98 Accession # Q62426 |
Classification | Monoclonal |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
Primary or Secondary | Primary |
Test Specificity | Detects mouse Cystatin B in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin A, C, D, E/M, F, S, SA, rhFetuin A, rhFetuin B, rhHPRG, or rhKininogen is observed. |
Clone | 227807 |
R&D Systems Human Cystatin B Antibody, R&D Systems™
Mouse Monoclonal Antibody has been used in 2 publications
R&D Systems Human/Rat Cystatin B Antibody, R&D Systems™
Goat Polyclonal Antibody has been used in 2 publications
Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
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Target Species | Human |
Host Species | Mouse |
Conjugate | Unconjugated |
Applications | Western Blot |
Form | Purified |
Isotype | IgG2b |
Gene Accession No. | P09228 |
Concentration | LYOPH |
Antigen | Cystatin SA |
Gene Symbols | CST2 |
Regulatory Status | RUO |
Purification Method | Protein A or G purified from hybridoma culture supernatant |
Dilution | Western Blot 1 ug/mL |
Gene Alias | CST2, CSTSA, cystatin 2, cystatin S5, cystatin SA, Cystatin-2, Cystatin-S5, cystatin-SA, cysteine-proteinase inhibitor, MGC71924, salivary cysteine (thiol) protease inhibitor |
Gene ID (Entrez) | 1470 |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Cystatin SA aa 21-141 Accession # P09228 |
Classification | Monoclonal |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
Primary or Secondary | Primary |
Test Specificity | Detects human Cystatin SA in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant human (rh) Cystatin S and SN and no cross-reactivity with rhCystatin A, B, C, D, E/M, rhFetuin A, B, and rhHPRG (Histidine-Proline-Rich Glycoprotein). |
Clone | 202332 |
R&D Systems Human Kininogen/Kininostatin Antibody, R&D Systems™
Goat Polyclonal Antibody has been used in 3 publications
Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
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Target Species | Human |
Host Species | Goat |
Conjugate | Unconjugated |
Applications | Western Blot |
Isotype | IgG |
Gene Accession No. | P01042 |
Concentration | LYOPH |
Antigen | Kininogen/Kininostatin/Bradykinin |
Gene Symbols | KNG1 |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Dilution | Western Blot 0.1 ug/mL |
Gene Alias | alpha 2-thiol Proteinase Inhibitor, Alpha-2-thiol proteinase inhibitor, BDK, bradykinin, Fitzgerald factor, High molecular weight kininogen, kininogen, kininogen 1, kininogen-1, KNG1, KNGHMWK, Williams-Fitzgerald-Flaujeac factor |
Gene ID (Entrez) | 3827 |
Immunogen | E. coli-derived recombinant human Kininostatin Lys438-Ser531 Accession # P01042 |
Classification | Polyclonal |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. |
Primary or Secondary | Primary |
Test Specificity | Detects human Kininogen/Kininostatin in direct ELISAs and Western blots. |
R&D Systems Human Cystatin B Antibody, R&D Systems™
Mouse Monoclonal Antibody has been used in 2 publications