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The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Format | 12-well Strips,8-well Strip,96- or 384-well Plate |
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Product Type | Sequencing Buffer |
For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
Technique | Fluorescent Dye Terminator Sequencing |
Product Line | BigDye™ Terminator |
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.
This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.
Shipping Condition | Room Temperature |
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Buffer | Sequencing Buffer |
Content And Storage | Store at 2°C to 8°C. |
Form | Liquid |
Concentration | 5X |
For Use With (Application) | Standard Sequencing |
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.
The GeneScan™ 1200 LIZ™ Size Standard is a fifth dye (LIZ™) labeled high density size standard for the reproducible sizing of fragment analysis samples.
A radio frequency identification tag is built into the frame for tracking critical run information, which is then reported on the software dashboard.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Sanger Sequencing Kit includes all reagents needed for sample preparation for sanger sequencing
A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.
Content And Storage | Room temperature (18 - 25 °C) |
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Final Product Type | Transfection-grade plasmid DNA |
Size | < 15 kbp |
Isolation Technology | Silica Membrane and Endotoxin-reduction Technology |
Purity | A260/A280: 1.80 - 1.85 |
Sample Volume | Up to 5 mL |
For Use With (Application) | Rapid purification of plasmids for transfection |
Test Time | 14 min/6 preps |
Includes | NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A |
Format | Mini Spin Column |
For Use With (Equipment) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Efficiency | Binding capacity: 35 μg |
Sample Type | Bacterial Culture (E. coli, High-copy Plasmids) |
Yield | 15 - 30 μg |
Shelf Life | At least 1 year |
Elution Volume | Elution Volume: 30 - 50 μL |
Bacterial Endotoxins | <50 EU/ng DNA |