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Optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins
To analyze cells for expression of cell surface and intracellular pluripotency markers and differentiation markers
For high purity isolation of hematopoietic progenitor and stem cells (HPCs and HSCs) from bone marrow
Analyzes populations of live and fixed cells for expression of pluripotency markers and differentiation markers and to sort live populations of pluripotent stem cells and their derivatives
For sorting and analysis of induced human pluripotent stem cell (hiPSC) cultures and cells in the process of being reprogrammed
Measuring expression of the stem cell master regulators'Nanog, Oct3/4, and Sox2'in heterogenous stem cell populations
To verify multipotency of mouse mesenchymal stem cells by in vitro functional differentiation
For reverse transcription of RNA without poly(A) tails, before qPCR or RNA sequencing
For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing