Protein Labeling Reagents
Protein Labeling Reagents
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Thermo Scientific™ EZ-Link™ Maleimide Protein Labeling Kit
A convenient method to label proteins with varying amounts of maleimide for crosslinking or conjugation with a thiol/sulfhydryl containing moiety. Sulfo-SMCC reacts with primary amines on a protein to form a covalently attached maleimide group that can be further reacted with a thiol/free cystine.
Cytiva Amersham™ QuickStain Protein Labeling Kit
Amersham QuickStain enables protein detection directly after SDS-PAGE or transfer. Supports Western blot normalization for quantitative Western blotting.
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Content And Storage | Store desiccated at -20°C, protect from moisture |
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Description | Pierce NHS-Fluorescein |
Product Type | NHS-Fluorescein |
Label or Dye | FITC (Fluorescein) |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 494 |
Label Type | Classic Dyes |
Chemical Reactivity | Amine |
Product Line | Pierce™ |
Thermo Scientific™ NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Cell Permeability | Cell-Impermeant |
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Content And Storage | Store at -20°C. |
Product Type | NHS-PEG4-Biotin |
Label or Dye | Biotin |
Solubility | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
Chemical Reactivity | Amine |
Product Line | EZ-Link™ |
Thermo Scientific™ DyLight™ 488 NHS Ester
Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.
Labeling Target | Proteins |
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Content And Storage | Store desiccated at -20°C, protected from light. |
Description | DyLight 488 NHS Ester |
Product Type | Dye |
Label or Dye | DyLight™ 488 |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 493 |
Label Type | DyLight Fluors |
Chemical Reactivity | Amine |
Product Line | DyLight™ |
Thermo Scientific™ DyLight™ 650 NHS Ester
Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.
Labeling Target | Proteins |
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Content And Storage | Store desiccated at -20°C. |
Description | DyLight 650 NHS Ester |
Product Type | Dye |
Label or Dye | DyLight™ 650 |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 672 |
Excitation | 652 |
Label Type | DyLight Fluors |
Chemical Reactivity | Amine |
Product Line | DyLight™ |
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Cell Permeability | Cell-Impermeant |
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Product Type | Sulfo-NHS-LC-Biotin |
Spacer | Mid-length |
Label or Dye | Biotin |
Solubility | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
Chemical Reactivity | Amine |
Product Line | EZ-Link™ |
Thermo Scientific™ Pierce™ Dye Removal Columns
Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.
Thermo Scientific™ FITC (5/6-fluorescein isothiocyanate), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Content And Storage | Store desiccated at -20°C, protect from moisture |
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Product Type | FITC |
Label or Dye | FITC (Fluorescein) |
Reactive Moiety | Isothiocyanate |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 494 |
Label Type | Classic Dyes |
Chemical Reactivity | Amine |
Product Line | Pierce™ |
Label Type | Biotin & Analogs |
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Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.
Chemical Reactivity | Amine |
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Thermo Scientific™ EZ-Link™ Maleimide-PEG2-Biotin, No-Weigh™ Format
Biotinylate reduced cysteines with this short, maleimide-activated, sulfhydryl-reactive biotinylation reagent containing a 2-unit PEG spacer arm to enhance water solubility.
Cell Permeability | Cell-Impermeant |
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Product Type | Maleimide-PEG2-Biotin |
Label or Dye | Biotin |
Solubility | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
Reactive Moiety | Maleimide |
Chemical Reactivity | Thiol |
Product Line | EZ-Link™ |
Thermo Scientific™ DyLight™ 488 Maleimide
Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin
Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Cell Permeability | Cell-Impermeant |
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Product Type | Sulfo-NHS-Biotin |
Spacer | Short |
Label or Dye | Biotin |
Solubility | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
Chemical Reactivity | Amine |
Product Line | EZ-Link™ |