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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Brown-to-purple (A595nm), ready-to-use, fast reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
High-quality, calibrated BSA solutions (2 mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent used to quickly measure total protein concentration compared to a protein standard.
Assay | Bradford Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
Sufficient For | 630 Tube Assays or 3160 Microplate Assays |
Quantity | 950 mL |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Quickly and reliably measure protein concentration of cell lysates or other samples that contain Triton™ X-100, NP-40 or other detergents using the the Thermo Scientific™ Pierce™ Detergent Compatible Bradford Assay Kit.
Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.
Type | Transfection Reagent |
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Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
The Pierce Dilution-Free Rapid Gold BCA Protein Assay Kit is an effortless, fast, and accurate assay optimized to determine total protein concentration. It is detergent-compatible and has a 5X wider dynamic range than traditional protein assays of 0.02–10 mg/mL.
Shipping Condition | Wet Ice |
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Specificity | Not Target-specific |
For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
Assay Range | 20 to 10,000 μg/mL |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ Dilution-Free™ |
Detection Method | Colorimetric |
Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
Substrate | D-Luciferin |
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Assay | Reporter Enzyme, Luciferase Reporter Assay |
Substrate Properties | Chemical Substrate |
Target | Luciferase, Firefly Luciferase |
Type | Assay Kit |
Format | 384-well plate, 96-well plate |
For Use With (Equipment) | Luminometer (Microplate) |
Compatible Cells | Mammalian Cells |
Substrate Type | Luciferase Substrate |
Technique | Enhanced Chemiluminescence |
Label Type | Enzyme Labeled |
Detection Method | Bioluminescence |
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.