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Protein Purification

Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,575)
Magnetic Affinity Separation Particles (179)
Protease Inhibitors and Phosphatase Inhibitors (170)
Spin Columns and Gravity-flow Columns (118)
Miscellaneous Immobilization Supports (110)
Gel Filtration Media and Columns (109)
Cell Lysis Detergents (74)
Affinity Gels (35)
Biotin-Binding Affinity Resins and Beads (30)
Miscellaneous Immobilized Affinity Ligands (24)
In vitro Protein Expression Systems (20)
Cell Lysis Enzymes (19)

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1 – 15 of 1,555 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Promotions are available
EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2

Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.

EDGE
Form Liquid
Reagent Type Detergent Solution
Product Line Surfact-Amps™
3

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
4

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

EDGE
5

Thermo Scientific™ Pepstatin A Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

EDGE
6

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
7

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

EDGE
8

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

EDGE
Particle Size 75μm
Buffer Aqueous Buffer
Ligand Type N-benzyl-n-methyl Ethanolamine
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type HiTrap Capto adhere column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Easy screening and convenient process development
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
9

Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

10

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

11

Thermo Scientific™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

EDGE
12

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Shipping Condition Wet Ice
Assay End-point Chromogenic Assay
Type Endotoxin Quantitation Kit
Target Endotoxins
Content And Storage • Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromogenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.
Format Kit
Assay Sensitivity 0.01 EU/mL
Assay Range 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line Pierce™
Detection Method Colorimetric
13

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur™
14

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

EDGE
Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor
Form Liquid
Product Type Protease Inhibitor Cocktail
Reagent Type Protease Inhibitor
Product Line Halt™
15

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

EDGE
Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor, Phosphatase Inhibitor
Form Liquid
Product Type Protease and Phosphatase Inhibitor Cocktail
Reagent Type Protease Inhibitor, Phosphatase Inhibitor
Product Line Halt™