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Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Shipping Condition | Dry Ice |
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Polymerase | phi29 DNA Polymerase |
Product Type | Stand-alone enzyme |
Reaction Time | 4 hours |
Concentration | 10 U/μL |
Optimal Reaction Temperature | 30°C |
For Use With (Application) | MDA-WGA,RCA |
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Shipping Condition | Dry Ice |
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Polymerase | EquiPhi29 DNA Polymerase |
Format | Kit |
Form | Liquid |
Product Type | DNA Amplification Kit |
Reaction Time | Up to 2 hours |
Research Category | Nucleic Acid Analysis,In Vitro Transcription,Cell-free Protein Expression,In Situ Sequencing,SNP Detection |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | RCA,MDA-WGA |
Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.
The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.
Includes | LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA |
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Detection Limit | <250 copies/25 μL (Direct LAMP) <100 copies/25 μL (Purified RNA-based LAMP) |
Bead Type | Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads |
For Use With (Equipment) | Heating block |
Enzyme | SuperScript IV & proprietary Bst-derived DNA polymerase |
Formulation | Liquid. 5 components included in the kit. |
Sample Type | Saliva or Nasopharyngeal/Nasal Swab |
Color | During reaction color changes from purple to blue |
Sample Volume | Range of sample inputs |
Key Functions | Detects SARS-CoV-2 presence |
For Use With (Application) | SARS-CoV-2 surveilance |
Detection Method | Colorimetric readout |