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1 – 15 of 25 results
1

Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
2

Magnesium Chloride Hexahydrate, Fisher BioReagents

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3

Cytiva Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

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4

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

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Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
5

Polysorbate 20, Fisher BioReagents™

Non-ionic detergent

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Water 3% max.
Viscosity 400 mPa/s at 25°C
Boiling Point 100°C
Ignition Residue 0.25% max.
Molecular Weight (g/mol) 522.68
Hydroxyl Value 96 to 108
Color Amber
Physical Form Viscous Liquid
Chemical Name or Material Polysorbate 20
SMILES CCCCCCCCCCCC(=O)OCCOCC(OCCO)C1OCC(OCCO)C1OCCO
Identification Pass Test
InChI Key HMFKFHLTUCJZJO-UHFFFAOYNA-N
ChemAlert Storage Symbol Gray
Density 1.100g/cm³
PubChem CID 443314
CAS 9005-64-5
Health Hazard 3 Emergency Overview
May cause eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. Obtain medical attention. .

NFPA

Health:1
Flammability:1
Instability:0
MDL Number MFCD00165986
Health Hazard 2 CAUTION!
pH 6
Synonym Polyoxyethylene-20-sorbitan Monolaurate,Polyoxyethylenesorbitan monolaurate
Recommended Storage RT
IUPAC Name 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate
Molecular Formula (C2H4O)y(C2H4O)w(C2H4O)x(C2H4O)zC18H34O6
6

Sodium Chloride, Fisher BioReagents™

CAS: 7647-14-5 Molecular Formula: ClNa Molecular Weight (g/mol): 58.44 MDL Number: MFCD00003477 InChI Key: FAPWRFPIFSIZLT-UHFFFAOYSA-M Synonym: sodium chloride,salt,table salt,halite,saline,rock salt,common salt,sodium chloride nacl,dendritis,purex PubChem CID: 5234 ChEBI: CHEBI:26710 SMILES: [Na+].[Cl-]

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PubChem CID 5234
CAS 7647-14-5
Molecular Weight (g/mol) 58.44
ChEBI CHEBI:26710
MDL Number MFCD00003477
SMILES [Na+].[Cl-]
Synonym sodium chloride,salt,table salt,halite,saline,rock salt,common salt,sodium chloride nacl,dendritis,purex
InChI Key FAPWRFPIFSIZLT-UHFFFAOYSA-M
Molecular Formula ClNa
7

Water (Biotech Grade), Fisher BioReagents

CAS: 7732-18-5 Molecular Formula: H2O Molecular Weight (g/mol): 18.015 InChI Key: XLYOFNOQVPJJNP-UHFFFAOYSA-N Synonym: dihydrogen oxide,dihydrogen monoxide PubChem CID: 962 ChEBI: CHEBI:15377 IUPAC Name: oxidane SMILES: O

PubChem CID 962
CAS 7732-18-5
Molecular Weight (g/mol) 18.015
ChEBI CHEBI:15377
SMILES O
Synonym dihydrogen oxide,dihydrogen monoxide
IUPAC Name oxidane
InChI Key XLYOFNOQVPJJNP-UHFFFAOYSA-N
Molecular Formula H2O
8

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

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9

Cytiva HiTrap™ Desalting Prepacked Columns

For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.

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Disposable Reusable
Particle Size >20 μm (dry), 15 to 88 μm (wet)
Content And Storage 4°C to 30°C in 20% Ethanol
Diameter (English) Inner Column 0.62 in.
Description HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.
Volume (Metric) Bed 5 mL
For Use With (Application) For rapid convenient desalting and buffer exchange
Diameter (Metric) Inner Column 16 mm
10

Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Particle Size 90μm
Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Flow Rate 150 to 600cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type Ni Sepharose excel chromato graphy media
dimMatrix Ni-Sepharose
Support Type 6% Highly Cross-linked Agarose
11

Thermo Scientific™ Savant™ SpeedVac™ Integrated Vacuum Concentrator Systems and Kits

Get all the necessary components for HPLC fractions with water/0.1% trifluoroacetic acid and reversed-phase SPE eluants applications with these kits.

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Chamber Material PTFE-coated Aluminum
Amperage 8 A
Temperature Range (Metric) Ambient to 80°C
Max. Vacuum <10 Torr (13 mbar, 1.3k Pa)
12

Cytiva His SpinTrap™ Spin Columns

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

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Content And Storage 4°C to 30°C
Support Type 6% Highly Cross-linked Agarose
13

Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

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Particle Size 45 to 165 μm
Type Pre-packed Column
Content And Storage 4°C to 30°C
pH Range 3 to 12
Material Polypropylene
Support Type 6% Cross-linked Agarose
14

Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Promotions are available
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
15

Cytiva Superdex 75 Increase Prepacked Column, 10/300 GL

Use these columns in preparative purification as well as for characterization and analysis of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva HisTrap™ excel Prepacked Columns feature tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.

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