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Thermo Scientific™ Proteinase K Powder (≥30 U/mg)
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.
Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ 8M Guanidine-HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Shipping Condition | Wet Ice |
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Content And Storage | Upon receipt store desiccated at -20°C. |
Description | EDC |
Cleavable | No |
Water Soluble | Yes |
Form | Powder |
Molecular Weight (g/mol) | 191.7 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Carboxyl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 Å) |
Reactive Moiety | Carbodiimide |
Spacer Arm Length | 0.0 Å |
PEGylated | No |
Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Optimize applications where product integrity and risk minimization are paramount with this high-quality formulation of carbodiimide crosslinker.
Content And Storage | Upon receipt store desiccated at -20°C. |
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Description | EDC |
Cleavable | No |
Water Soluble | Yes |
Molecular Weight (g/mol) | 191.7 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Carboxyl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 Å) |
Reactive Moiety | Carbodiimide |
Spacer Arm Length | 0.0 Å |
Product Line | Pierce™ |
PEGylated | No |
Description | DSG |
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Cleavable | No |
Water Soluble | No |
Form | Powder |
Molecular Weight (g/mol) | 326.26 |
Solubility | DMF, DMSO |
Labeling Method | Chemical Labeling |
Crosslinker Type | Homobifunctional |
Chemical Reactivity | Amine-Amine |
Cell Permeability | Yes |
Format | Standard, Single-use |
Product Type | Crosslinker |
Spacer | Short (<10 Å) |
Reactive Moiety | NHS Ester |
Spacer Arm Length | 7.7 Å |
PEGylated | No |
Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Molecular Weight (g/mol) | 217.13 |
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Solubility | Water |
PEGylated | No |
Thermo Scientific™ Pierce™ Biotin Quantitation Kit
HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.
Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Label Type | Biotin & Analogs |
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Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Shipping Condition | Ambient |
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Content And Storage | Upon receipt store desiccated at -20°C. |
Description | Sulfo-SMCC |
Cleavable | No |
Water Soluble | Yes |
Molecular Weight (g/mol) | 436.37 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Sulfhydryl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 Å) |
Reactive Moiety | Maleimide, Sulfo-NHS Ester |
Spacer Arm Length | 8.3 Å |
PEGylated | No |
Thermo Scientific™ Pierce™ DTT (Dithiothreitol)
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.
Thermo Scientific™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.