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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
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Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
Shipping Condition | Dry Ice |
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Polymerase | Phusion Plus DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >100X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 sec./kb |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima H Minus |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | RevertAid H Minus |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C to 45°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ GeneJET RNA Purification Kit
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
Shipping Condition | Room Temperature |
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Final Product Type | Total RNA |
Sample Type | Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions |
Isolation Technology | Silica Spin Column |
Starting Material Amount | Bacteria: ≤109 cells Blood: 0.5 mL Cells: ≤107 Tissue and Insects: 30 mg Yeast: ≤4 x 108 cells Enzymatic reactions: ≤100 μL |
Purification Time | 15 min. (After sample homogenization) |
Yield | Up to 160 μg |
Scale | Mini |
Elution Volume | 50–100 μL |
For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ RevertAid RT Reverse Transcription Kit
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.
First Strand cDNA Synthesis Kit
Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.
Applied Biosystems™ 3730 Running Buffer (10X)
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.
Thermo Scientific™ MagJET Separation Rack, 12 x 1.5 mL tube
The MagJET separation rack allows fast and efficient procedures for magnetic bead-based nucleic acid isolation and affinity purification workflows.
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
Polymerase | Fast DNA Polymerase |
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Reverse Transcriptase | M-MLV |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
Form | Liquid |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Concentration | 4X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Thermo Scientific™ dCTP Solution (100 mM)
Thermo Scientific dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Applied Biosystems™ GeneScan™ 1200 LIZ™ dye Size Standard
The GeneScan™ 1200 LIZ™ Size Standard is a fifth dye (LIZ™) labeled high density size standard for the reproducible sizing of fragment analysis samples.
Applied Biosystems™ BigDye™ Terminator v1.1 & v3.1 5X Sequencing Buffer
This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.
Shipping Condition | Room Temperature |
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Buffer | Sequencing Buffer |
Content And Storage | Store at 2°C to 8°C. |
Form | Liquid |
Concentration | 5X |
For Use With (Application) | Standard Sequencing |