UserName
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientificâ„¢ Bovine Serum Albumin.
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientificâ„¢ 50X TAE Buffer (Tris-acetate-EDTA).
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Product Type | T4 DNA Ligase |
---|---|
Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
Product Type | RNase Inhibitor |
---|---|
Compatible Buffer | Storage Buffer |
Concentration | 40 U/μL |
Product Line | RiboLockâ„¢ |
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Form | Liquid |
---|---|
Product Type | Proteinase K |
Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientificâ„¢ 10X TBE Buffer (Tris-borate-EDTA).
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Product Type | Alkaline Phosphatase |
---|---|
Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Concentration | 1 U/μL |
Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Product Type | Exonuclease I |
---|---|
Enzyme | Exonuclease |
Concentration | 20 U/μL |
The Thermo Scientific NTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP.
Degrade single-stranded DNA and RNA with this endonuclease.