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  7. 16232931

IBA Lifesciences CD4 Fab-TACS™ Gravity Kit, Mouse

For manual positive cell separation. The Fab-TACS™ Gravity Kit provides label-free target cells without the use of high affinity antibodies and magnetic beads

Brand:  IBA Lifesciences 6-3401-010

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Product Code. 16232931

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Description

Description

  • The kit allows the selection of CD4+ T cells from mouse spleen.
  • Fab-TACS™ Gravity column (Capacity: 1x108target cells each)
  • CD4 Fab-Strep, mouse
  • Buffer CI (10x)
  • Biotin stock solution
  • Fab-TACS™ Gravity Adapter
  • The Fab-TACS™ Gravity column is filled with a Strep-Tactin™ coated matrix made of cell-grade agarose.
  • Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix.
  • Subsequently, single-cell tissue suspensions pass through the column.
  • Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell.
  • Non-target cells are efficiently washed away.
  • In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps.
  • After elution, the Fab-Streps self-dissociate from the cell surfaces.
  • The label-free authentic target cells are now ready for further downstream applications.
    • Specifications

    Specifications

    2°C to 8°C
    Non-magnetic Cell Isolation Kit
    1x108 target cells/column
    10 Columns
    Mouse
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