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Cytiva Con A Sepharose™ 4B Medium
An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids
Brand: Cytiva 17-0440-03
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Description
Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.
- Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
- Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Group-specific adsorbent for molecules containing sugars
- Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
- Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
- Average particle size: 90μm
- Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol
Specifications
| 30mg/mL | |
| 4% Agarose | |
| 4 to 9 | |
| 2°C to 8°C | |
| Concanavalin A |
| 13mg Con A/mL Drained Medium | |
| 90 μm | |
| 5 mL | |
| For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes |