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Invitrogen™ Cortisone Competitive ELISA Kit

Competitive ELISA

Brand:  Invitrogen™ EIACORX10

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Product Code. 17855217

  • 4635.00€ / Pack of 10
Estimated Shipment: 31-01-2025
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Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
This item is not returnable. View return policy

Description

Description

The Cortisone ELISA quantitates Cortisone in serum,saliva,urine,plasm,or dried fecal extracts.

Principle of the method

The Cortisone Competitive ELISA research-use-only kit is designed to quantitatively measure cortisone in extracted dried fecal samples,urine,saliva,plasma,and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two-hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Cortisone (Kendall’s Compound E) is a glucocorticoid which is the product of cortisol (Compound F) deactivation by 11-beta-HSD2 (hydroxysteroid dehydrogenase), prohibiting receptor activation. The balance between cortisone and cortisol glucocorticoids helps to regulate the anti-inflammatory response. Cortisone is identical across all species.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

97.66 to 100,000 pg/mL
Cortisone Competitive ELISA Kit
HRP
ELISA Kit
10 x 96 Assays
ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
11.9%
Pre-coated 96-well plate, Standard, Assay Diluent Concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
Fecal Extract (50 μL), Plasma (1 μL), Saliva (10 μL), Serum (1 μL), Urine (10 μL)
Chemical
2 hr. 30 min.
29 pg/mL
Multiplex Assay on the Luminex Platform
Fluorescence
Fecal Extract, Plasma, Saliva, Serum, Urine
Human
Colorimetric Microplate Reader
8.6%
HRP
Inflammation
-20°C
1 hr. 20 min.
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