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IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.
Brand: IBA Lifesciences 5-4145-001
This item is not returnable.
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Description
- The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
- The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
- The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
- Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Specifications
| Inducible with anhydrotetracycline | |
| Tet | |
| Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| His Tag (6x), Twin-Strep-tag | |
| 250 ng/μL | |
| 5 μg | |
| Vector for recombinant expression in E. coli |
| Ampicillin | |
| Vector | |
| E.coli | |
| Direct Cloning | |
| Forward | |
| pASG-IBA145 |