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IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.

266.00€ - 286.00€

Specifications

Inducing Agent	Inducible with anhydrotetracycline
Marker Gene	Ampicillin
Promoter	Tet
Product Type	Vector
Content And Storage	Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage

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Products 17

Product Code	Brand	Vector	Price	Quantity & Availability
Product Code	Brand	Vector	Price	Quantity & Availability
15603758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4164-001	pASG-IBA164	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

15613758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4167-001	pASG-IBA167	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

15623748 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4103-001	pASG-IBA103	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15623758 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4168-001	pASG-IBA168	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

15633728 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4000-001	pASG-IBAwt1	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15633738 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4043-001	pASG-IBA43	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15643748 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4105-001	pASG-IBA105	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15653738 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4045-001	pASG-IBA45	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15653748 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4123-001	pASG-IBA123	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

15663728 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4003-001	pASG-IBA3	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15663738 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4062-001	pASG-IBA62	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

15663748 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4143-001	pASG-IBA143	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15683728 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4005-001	pASG-IBA5	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15683738 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4064-001	pASG-IBA64	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

15683748 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4145-001	pASG-IBA145	286.00€ 5µg	Estimated Shipment: 17-01-2025 Log in to see stock.	Add to basket

15693728 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4023-001	pASG-IBA23	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

15693748 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4162-001	pASG-IBA162	This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance		N/A

Description

The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.

Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Inducing Agent	Inducible with anhydrotetracycline
Promoter	Tet
Content And Storage	Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Quantity	5 μg

Marker Gene	Ampicillin
Product Type	Vector
Concentration	250 ng/μL

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IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

Specifications

Description

Specifications

Documents