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Invitrogen™ Estrone-3-Glucuronide (E1G) Competitive ELISA Kit
Competitive ELISA
Brand: Invitrogen™ EIA17E3X10
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Estrone-3 Glucuronide (E1G) ELISA quantitates estrone-3 glucuronide in extracted serum,plasma,urine,dried fecal extracts or cell culture medium.
Principle of the method
The Estrone-3-Sulfate (E1S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Estrone-3-Sulfate (E1S) in a variety of matrices,including serum,plasma,urine,and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Estrone-3-glucuronide (E1G) is the principle secreted form of circulating estradiol in mammals. Estrone-3-glucuronide (E1G) is identical across all species.
Specifications
| 15.6 to 1000 pg/mL | |
| Estrone-3-Glucuronide (E1G) Competitive ELISA Kit | |
| HRP | |
| ELISA Kit | |
| 10 x 96 Assays | |
| ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti | |
| 5.6% | |
| Pre-coated 96-well plate, Standard, Assay Diluent Concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Fecal Extract (50 μL), Serum (50 μL), Supernatant (50 μL), Urine (6.25 μL) | |
| Chemical | |
| 2 hr. 30 min. |
| 7.38 pg/mL | |
| Multiplex Assay on the Luminex Platform | |
| Fluorescence | |
| Fecal Extract, Serum, Supernatant, Urine | |
| Human | |
| Colorimetric Microplate Reader | |
| 3.8% | |
| HRP | |
| Reproductive Biology | |
| -20°C | |
| 1 hr. 20 min. |