Thermo Scientific™ EZ-Link™ Alkoxyamine-PEG4-Biotin

Description

Thermo Scientific™ EZ-Link Alkoxyamine-PEG4-Biotin is a biotinylation reagent containing a four-unit polyethylene glycol (PEG) spacer for biotinylating macromolecules at carbohydrate groups that have been oxidized to form aldehydes.

Alkoxyamine-activated biotinylation reagents such as this one are useful for biotin-labeling glycoproteins and other molecules that have oxidizable polysaccharides groups. EZ-Link Alkoxyamine-PEG-Biotin reagents contain a multi-functional extended spacer arm that is a flexible, non-immunogenic hydrophilic polyethylene glycol (PEG), which imparts water solubility to labeled molecules. Consequently, antibodies labeled with biotin alkoxyamine reagents exhibit less aggregation when stored in solution compared to antibodies labeled with reagents having only hydrocarbon spacers.

Highlights:

• Biotin – label macromolecules for detection or affinity purification with avidin and streptavidin

• Alkoxyamine-activated – reacts with aldehyde groups (e.g., oxidized sugars in glycoproteins) to attach biotin by stable oxime bonds

• Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and improve the solubility of labeled antibodies

• Spacer arm – the reach between the point of attachment and the biotin affinity tag is nearly 27 angstroms, sufficient to minimizing steric hindrance for binding

Specifications:

• Chemical formula: C₁₈H₃₄N₄O₆S
• Molecular weight: 434.55
• Spacer arm: 27.0 angstroms
• Net mass added to target: 416.53
• Form: white solid
• Solubility: dissolve in DMSO to make 250mM stock