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Invitrogen™ GRO alpha (CXCL1) Human ELISA Kit

For the quantitative detection of human sIL-2R.

Brand:  Invitrogen™ BMS212-2

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Product Code. 17502206

  • 678.26€ / 1 set
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Includes:
  • Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sIL-2R
  • HRP-Conjugate anti-human sIL-2R monoclonal antibody
  • Human sIL-2R Standard lyophilized, 40ng/mL upon reconstitution
  • Sample Diluent
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  • Substrate Solution (tetramethyl-benzidine)
  • Stop Solution (1M Phosphoric acid)
  • Blue-Dye
  • Green-Dye
  • Adhesive Films
This item is not returnable. View return policy

Description

Description

The Human Soluble Interleukin 2 Receptor/CD25 (Hu IL-2R) ELISA quantitates Hu IL-2R in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-2R. Principle of the method The Human IL-2R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

P01589
0.27 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CD25,IDDM10,IL2R,IMD41,TCGFR,p55
7.2%
HRP
RUO
2°C to 8°C
Human
3 hr. 10 min.
0.31-20.0 ng/mL
HRP
Plasma, Serum, Supernatant
ELISA
3559
9.8%
Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
CD25 antigen, TAC, Interleukin-2 receptor alpha
1 hr. 20 min.
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