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Invitrogen™ GRO alpha (CXCL1) Human ELISA Kit
For the quantitative detection of human sIL-2R.
678.26€
Specifications
| Accession Number | P01589 |
|---|---|
| Assay Range | 0.31-20.0 ng/mL |
| Assay Sensitivity | 0.27 ng/mL |
| Conjugate | HRP |
| Product Type | ELISA Kit |
Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sIL-2R
HRP-Conjugate anti-human sIL-2R monoclonal antibody
Human sIL-2R Standard lyophilized, 40ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
Description
The Human Soluble Interleukin 2 Receptor/CD25 (Hu IL-2R) ELISA quantitates Hu IL-2R in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-2R. Principle of the method The Human IL-2R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.Specifications
| P01589 | |
| 0.27 ng/mL | |
| ELISA Kit | |
| Human | |
| Colorimetric Microplate Reader | |
| CD25,IDDM10,IL2R,IMD41,TCGFR,p55 | |
| 7.2% | |
| HRP | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
| CD25 antigen, TAC, Interleukin-2 receptor alpha | |
| 1 hr. 20 min. |
| 0.31-20.0 ng/mL | |
| HRP | |
| Plasma, Serum, Supernatant | |
| ELISA | |
| 3559 | |
| 9.8% | |
| Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| 2°C to 8°C | |
| Human | |
| 3 hr. 10 min. |
For Research Use Only