missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids
Brand: Cytiva 28-9356-07
View more versions of this product
This item is not returnable.
View return policy
Description
Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).
- Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
- Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
- Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
- Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)
Media
- Average particle size: 50μm
- Matrix: Allyl dextran and N, N'-methylenebisacrylamide
- pH Stability working range: 3 to 11
- pH Stability cleaning range: 2 to 13
- Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
- Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
- Material (bed support): Polymethyl-Methacrylate
HiPrep 16/60 Sephacryl S-400 HR
- Media:
- Fractionation [Mr] globular proteins: 2 x 104-8 x 106
- Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
- Fractionation range [Mr] DNA exclusion limit: 271bp
- Fractionation [Mr] Dextrans: 1 x 104-2 x 106
- Media:
- Exclusion limit for Dextrans: 100 x 106
- Fractionation range [Mr] DNA exclusion limit: 1078bp
- Fractionation [Mr] Dextrans: 4 x 104-2 x 107
Specifications
| Each | |
| 25 to 75 μm | |
| 320 mL | |
| 2.6cm | |
| 23.62 in. | |
| Polypropylene | |
| 3 to 11 | |
| Polysaccharides, macromolecules with extended structures and even small plasmids |
| <0.5 mL/min. | |
| Low to medium pressure AKTA™ systems or pumped chromatography systems | |
| Sephacryl HR Pre-packed Gel Filtration Column | |
| Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids | |
| 60cm | |
| 1.5 bar [0.15 MPa] (22 psi) | |
| Allyl Dextran and N,N'-methylenebisacrylamide | |
| 4°C to 30°C, 20% Ethanol |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HiPrep 26/60 Sephacryl S-500 HR
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol