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Cytiva His SpinTrap™ Spin Columns

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

453.00€ - 475.00€

Specifications

Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
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Products 2
Product Code Brand Type Price Quantity & Availability  
Product Code Brand Type Price Quantity & Availability  
11733319
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Cytiva
28-4013-53
Spin Column
453.00€
Pack of 50
In Stock
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10157094
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Cytiva
28-9321-71
Spin Column Kit
475.00€
1 L
Estimated Shipment: 22-01-2025
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Description

Description

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750μg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34μm

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

Specifications

Specifications

6% Highly Cross-linked Agarose
4°C to 30°C
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Skin sensitiser Category 1

Hazard Statement
  • H317-May cause an allergic skin reaction.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P501-Dispose of contents/container to .

Supplemental information
  • MIXTURE LIST-Contains : nicke, reaction massof:5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2Hisothiazol-3-one

SDS
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