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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose
Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
180.00€ - 9260.00€
Specifications
| Capacity (Metric) | 20 mg His-Tagged GFP Binding per mL Resin |
|---|---|
| Content And Storage | Store at 4°C |
| Form | Liquid Suspension |
| Product Line | HisPur™ |
| Target | His-Tagged Protein |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
13414199
|
Thermo Scientific™
25217 |
1 L |
9260.00€
1 L |
Estimated Shipment: 12-12-2024
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|
13484189
|
Thermo Scientific™
25214 |
10 mL |
180.00€
10mL |
In Stock
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|
13404199
|
Thermo Scientific™
25215 |
50 mL |
738.00€
50mL |
In Stock
Log in to see stock. |
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|
13494189
|
Thermo Scientific™
25216 |
250 mL |
2875.00€
250mL |
Estimated Shipment: 12-12-2024
Log in to see stock. |
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Description
Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
Additional Information and Online Ordering
HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.Highlights:
High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour
High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates
Versatile – can be used to purify proteins under native and denaturing conditions
Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour
Compatible – maintains function in a wide variety of chemicals and pH conditions
Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Medium- to large-scale FPLC purification of polyhistidine tagged proteins
Specifications
| 20 mg His-Tagged GFP Binding per mL Resin | |
| Liquid Suspension | |
| His-Tagged Protein | |
| Divalent Nickel, Metal Chelate | |
| Affinity, Agarose Resin |
| Store at 4°C | |
| HisPur™ | |
| Agarose | |
| Bottle |
For Research Use Only. Not for use in diagnostic procedures.