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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Brand: Cytiva 17-5248-02
This item is not returnable.
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Description
- Prepacked with Ni Sepharose™ High Performance medium
- Negligible nickel ion leakage
- Pressure Max: 3 bar
- Column material: polypropylene
- 1 and 5mL columns
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Manual operation with syringe, pump or chromatography system
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: 3 to 12
1mL columns
- Bed Dimensions: 7 x 25mm
- Flow velocity: 4mL/min.
5mL columns
- Bed Dimensions: 16 x 25mm
- Flow velocity: 5mL/min.
Specifications
| 5 mL | |
| 16 mm | |
| 4 mL/min. (1 mL), 5 mL/min. (5 mL) | |
| 3 to 12 | |
| 4°C to 30°C | |
| 5 x 5 mL |
| 0.6 in. | |
| HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. | |
| Polypropylene | |
| 6% Highly Cross-linked Agarose | |
| 5 bar (0.5 MPa, 70 psi) | |
| Pre-packed Column |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : Ethanol, Nickel
- UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.