For the quantitative detection of human IL-9.
Brand: Invitrogen™ BMS2081
The Human Interleukin-9 (Hu IL-9) ELISA quantitates Hu IL-9 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-9. Principle of the method The Human IL-9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta.
| P15248 | |
| 0.5 pg/mL | |
| ELISA Kit | |
| Human | |
| Colorimetric Microplate Reader | |
| HP40,IL-9,P40 | |
| 5.3% | |
| HRP | |
| RUO | |
| 2°C to 8°C | |
| Human | |
| 3 hr. 30 min. |
| 3.1-200 pg/mL | |
| Biotin | |
| Plasma, Serum, Supernatant | |
| ELISA | |
| 3578 | |
| 4.8% | |
| Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 96 Tests | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
| Interleukin 9, MEA, HP40, P40 cytokine | |
| 1 hr. 20 min. |
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