Invitrogen™ Novex™ TNF-alpha Human Ultrasensitive Magnetic Bead Kit

Description

By mixing this Ultrasensitive kit with other targets from the Ultrasensitive kit portfolio, more data can be obtained from each sample, saving both money and time compared to more traditional methods (such as ELISA). This assay for employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The assay is suitable for use with the Luminex™ 200™, FLEXMAP 3D™, and MAGPIX™ systems.

• Superior performance—Accurate, reproducible, and sensitive quantitation of multiple proteins
• High quality—Fully qualified antibodies produce excellent specificity and sensitivity
• Fast and easy protocols—Perform your assays and analyze your data typically in less than one day

Luminex™ xMAP™ technology—effective and proven analytical tool
The Human TNF-α Ultrasensitive Magnetic Bead Kit is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex™ assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead has a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).

Magnetic beads let you do more with your time
Magnetic bead-based assays enable automation, decrease hands-on time, and increase throughput and precision. Using the fast and easy Luminex™ protocols, you can obtain results in as little as 3.5 hours. This line of assays was carefully designed and tested for maximum sensitivity, range, and correlation. Using the same components as our polystyrene bead-based assays allows for performance with identical quality and consistency. Standard curves generated with this assay system extend over several orders of magnitude of reagent concentration, while the sensitivity and quality of the assays are comparable to ELISAs and our polystyrene bead-based assays, enabling superior performance.

More validation for confidence in your results
The Human TNF-α Ultrasensitive Magnetic Bead Kit has been validated using the same rigorous criteria that distinguishes Luminex™ assays from other commercially available assays. Our fully qualified antibodies help ensure the specificity and sensitivity of our assays. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay.