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Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
Brand: Cytiva 17-5318-03
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Description
Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
- Designed for scaling up purification of histidine-tagged proteins.
- High protein binding capacity and minimal leakage of Ni2+ ions.
- As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep and HisTrap column formats.
- Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
- The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
- Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
- As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Specifications
| 0.1M HCl, 0.1M NaOH, 8M Urea | |
| 500mL | |
| 250mm | |
| 1 bar | |
| 90μm | |
| 4° to 30°C |
| Ni Sepharose 6 FF chromatography media | |
| 9.84 in. | |
| 600cm/hr. | |
| 3 to 12 | |
| 6% Cross-linked Agarose | |
| Ni-Sepharose |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- Warning
Hazard Category
- Flammable liquid Category 3
- ACUTE AQUATIC Acute 1
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H400-Very toxic to aquatic life.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P273-Avoid release to the environment.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.