Macherey-Nagel™ NucleoBond™ AXG 500 Columns

Description

Genomic DNA quality is extraordinarily high. Samples are first lysed under special conditions for removal of erythrocytes. After incubation with proteinase K, the sample is diluted with buffer N2 before loading onto the NucleoBond™ column. After elution with high-salt buffer the genomic DNA is precipitated with isopropanol. The DNA purified by this method is suitable for all kinds of subsequent reactions. Kit components: NucleoBond™ CB 100 kit - NucleoBond AXG 100 columns, buffers, proteinase K. NucleoBond™ CB 500 kit - NucleoBond™ AXG 500 columns, buffers, proteinase K.

• Anion exchange technology
• Format: gravity flow columns
• Sample material: whole blood, buffy coat, cultured cells (up to 20mL blood)
• Fragment size: 500bp to 300kb
• Yield: 100μg or 500μg depending on column used
• Binding capacity: 100μg or 500μg depending on column used
• Preparation time: 4 to 5hrs For purification of up to 500μg genomic DNA from whole blood.