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Macherey-Nagel™ NucleoSnap cfDNA kit for cell-free DNA from plasma
Isolation of cell-free DNA from up to 10 mL blood plasma, utilizing a new column design for high-speed vacuum processing (e.g. with the NucleoVac 24 Vacuum Manifold). Easy-to-use snap-off columns produce ready-to-use, transfection- and molecular-grade DNA with no alcohol precipitation necessary.
Brand: Macherey-Nagel™ 740300.10
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Description
The NucleoVac 24 Vacuum Manifold allows the processing of silica columns for nucleic acid purification by vacuum.
- Snap-off column design for quick processing of large sample volumes
- Compatible with the NucleoVac 24 vacuum manifold (sold separately, MPN 740299
- No need for carrier-RNA
- Process: DNA precipitation—filtration—washing—elution
- Optimized protocol for use with EDTA or Cell-Free DNA BCT™ (Streck) blood draw tubes
- Highly efficient recovery of cell-free DNA from Liquid Biopsies
- No alcohol precipitation necessary
- Typical downstream applications: NGS, qPCR, ddPCR
Specifications
| Snap-off Column | |
| Plasma, Urine | |
| Cell-free DNA | |
| DNA isolation from blood plasma | |
| 10 | |
| 1 - 5 mL | |
| Depends on sample source, storage, and quality |
| Silica Membrane Technology | |
| Elution volume: 20 - 100 μL | |
| Vacuum (e.g. NucleoVac 24 Vacuum Manifold and vacuum pump) | |
| NucleoSnap DNA Plasma Columns, Collection Tubes, buffers, Proteinase K | |
| Fragment size: ≥ 50 bp | |
| 45 min/6 preps |
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Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- NucleoSnap DNA Plasma
Signal Word
- Danger
Hazard Category
- Flammable liquid Category 2
- Flammable liquid Category 3
- Substances and mixtures corrosive to metals Category 1
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H226-Flammable liquid and vapour.
- H290-May be corrosive to metals.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
Supplemental information
- MIXTURE LIST-Contains : potassium hydroxidesolution/proteinaseK, liquid (origin: tritirachium album)/guanidine hydrochlori
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Product Title
Macherey-Nagel™ NucleoSnap cfDNA kit for cell-free DNA from plasma > 10 Preps
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